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KMID : 0613820080180101442
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Journal of Life Science
2008 Volume.18 No. 10 p.1442 ~ p.1446
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Genetic Relationship between Populations and Analysis of Genetic Structure in Hanwoo Proven and Regional Area Populations
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Oh Jae-Don
Jeon Gwang-Joo
Lee Hak-Kyo
Cho Byung-Wook
Lee Mi-Rang
Kong Hong-Sik
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Abstract
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Seven populations of 586 Hanwoo have been characterized by using 10 microsatellite DNA markers. Size of microsatellite markers decided using GeneMapper Software (v.4.0) after analyze in kinds of ABI machine of name of 3130. Frequencies of microsatellites markers were used to estimate heterozygosities and genetic distances. Genetic distances between populations were obtained using Nei’s DA distance method. Expected heterozygosity between each population was estimated very analogously. Genetic distances (0.0413) between Kangwan (KW) and Gyonggi (GG), Jeonpuk (JP) were nearest than distances between other populations by 0.021. Genetic distances between Gyonggi (GG) and Kyongpuk (KP) showed far distance than other populations by 0.032. In the UPGMA tree that is made based on DA distance matrix. Each individuals were not ramified to different group and were spread evenly in phylogenetic dendrogram about all Hanwoo of each regional area populations. But Hanwoo proven population was ramified to different group.
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KEYWORD
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Hanwoo, genetic relationship, microsatellite marker
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